anthony leug Search Results


anthony leug  (Addgene inc)
93
Addgene inc anthony leug
Anthony Leug, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anthony leug/product/Addgene inc
Average 93 stars, based on 1 article reviews
anthony leug - by Bioz Stars, 2026-06
93/100 stars
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psicheck3 vector  (Addgene inc)
93
Addgene inc psicheck3 vector
Regulation of FOS translation efficiency by ebselen. ( a ) Schematic representation of the <t>psiCHECK3-FO</t> translation reporter construct (upper) and Integrated Genomics Viewer plot showing m 6 A peaks around FOS mRNA in MCF7 cells (data from GSM5368774 , retrieved from https://www.ncbi.nlm.nih.gov/geo/ (accessed on 2 May 2025)). The sequence of the m 6 A methylation site within the FOS 3′ untranslated region is highlighted. The asterisk symbol (*) indicates the methylated adenosine. ( b ) Renilla luciferase ( RLuc ) activity was measured in cell lysates from MCF7 cells transfected with the psiCHECK3-FOS construct and treated with ebselen. RLuc activity was normalized to Firefly luciferase activity ( FLuc ) ( upper panel ), and the corresponding mRNA expression in the lysates was analyzed using end-point PCR for the indicated target genes ( lower panel ). Data are presented as mean ± SD; n = 3. One-way ANOVA, ** , ## p < 0.01. ( c , d ) Ebselen reduces c-Fos translation efficiency. MCF7 cells were cultured in serum-free medium with indicated concentrations of ebselen for 24 h, followed by puromycin pretreatment for 1 h and EGF treatment for another 1 h. Whole cell lysates (WCLs) were analyzed via immunoprecipitation/immunoblotting (IP/IB) using the indicated antibodies ( c ). MCF7 cells transfected with sgControl or sgYTHDF1 were cultured in serum-free medium for 24 h, followed by pretreatment with puromycin for 1 h and EGF stimulation for another 1 h. WCLs were analyzed via IP/IB using the indicated antibodies ( d ).
Psicheck3 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psicheck3 vector/product/Addgene inc
Average 93 stars, based on 1 article reviews
psicheck3 vector - by Bioz Stars, 2026-06
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peptag pka reaction buffer containing 0.4 lg/ll kemptide peptag a1 (l-r-r-a-s-l-g)  (Promega)
90
Promega peptag pka reaction buffer containing 0.4 lg/ll kemptide peptag a1 (l-r-r-a-s-l-g)
Regulation of FOS translation efficiency by ebselen. ( a ) Schematic representation of the <t>psiCHECK3-FO</t> translation reporter construct (upper) and Integrated Genomics Viewer plot showing m 6 A peaks around FOS mRNA in MCF7 cells (data from GSM5368774 , retrieved from https://www.ncbi.nlm.nih.gov/geo/ (accessed on 2 May 2025)). The sequence of the m 6 A methylation site within the FOS 3′ untranslated region is highlighted. The asterisk symbol (*) indicates the methylated adenosine. ( b ) Renilla luciferase ( RLuc ) activity was measured in cell lysates from MCF7 cells transfected with the psiCHECK3-FOS construct and treated with ebselen. RLuc activity was normalized to Firefly luciferase activity ( FLuc ) ( upper panel ), and the corresponding mRNA expression in the lysates was analyzed using end-point PCR for the indicated target genes ( lower panel ). Data are presented as mean ± SD; n = 3. One-way ANOVA, ** , ## p < 0.01. ( c , d ) Ebselen reduces c-Fos translation efficiency. MCF7 cells were cultured in serum-free medium with indicated concentrations of ebselen for 24 h, followed by puromycin pretreatment for 1 h and EGF treatment for another 1 h. Whole cell lysates (WCLs) were analyzed via immunoprecipitation/immunoblotting (IP/IB) using the indicated antibodies ( c ). MCF7 cells transfected with sgControl or sgYTHDF1 were cultured in serum-free medium for 24 h, followed by pretreatment with puromycin for 1 h and EGF stimulation for another 1 h. WCLs were analyzed via IP/IB using the indicated antibodies ( d ).
Peptag Pka Reaction Buffer Containing 0.4 Lg/Ll Kemptide Peptag A1 (L R R A S L G), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peptag pka reaction buffer containing 0.4 lg/ll kemptide peptag a1 (l-r-r-a-s-l-g)/product/Promega
Average 90 stars, based on 1 article reviews
peptag pka reaction buffer containing 0.4 lg/ll kemptide peptag a1 (l-r-r-a-s-l-g) - by Bioz Stars, 2026-06
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kemptide (l-r-r-a-s-l-g)  (Promega)
90
Promega kemptide (l-r-r-a-s-l-g)
Regulation of FOS translation efficiency by ebselen. ( a ) Schematic representation of the <t>psiCHECK3-FO</t> translation reporter construct (upper) and Integrated Genomics Viewer plot showing m 6 A peaks around FOS mRNA in MCF7 cells (data from GSM5368774 , retrieved from https://www.ncbi.nlm.nih.gov/geo/ (accessed on 2 May 2025)). The sequence of the m 6 A methylation site within the FOS 3′ untranslated region is highlighted. The asterisk symbol (*) indicates the methylated adenosine. ( b ) Renilla luciferase ( RLuc ) activity was measured in cell lysates from MCF7 cells transfected with the psiCHECK3-FOS construct and treated with ebselen. RLuc activity was normalized to Firefly luciferase activity ( FLuc ) ( upper panel ), and the corresponding mRNA expression in the lysates was analyzed using end-point PCR for the indicated target genes ( lower panel ). Data are presented as mean ± SD; n = 3. One-way ANOVA, ** , ## p < 0.01. ( c , d ) Ebselen reduces c-Fos translation efficiency. MCF7 cells were cultured in serum-free medium with indicated concentrations of ebselen for 24 h, followed by puromycin pretreatment for 1 h and EGF treatment for another 1 h. Whole cell lysates (WCLs) were analyzed via immunoprecipitation/immunoblotting (IP/IB) using the indicated antibodies ( c ). MCF7 cells transfected with sgControl or sgYTHDF1 were cultured in serum-free medium for 24 h, followed by pretreatment with puromycin for 1 h and EGF stimulation for another 1 h. WCLs were analyzed via IP/IB using the indicated antibodies ( d ).
Kemptide (L R R A S L G), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/kemptide (l-r-r-a-s-l-g)/product/Promega
Average 90 stars, based on 1 article reviews
kemptide (l-r-r-a-s-l-g) - by Bioz Stars, 2026-06
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anthony leung 32  (Addgene inc)
93
Addgene inc anthony leung 32
Regulation of FOS translation efficiency by ebselen. ( a ) Schematic representation of the <t>psiCHECK3-FO</t> translation reporter construct (upper) and Integrated Genomics Viewer plot showing m 6 A peaks around FOS mRNA in MCF7 cells (data from GSM5368774 , retrieved from https://www.ncbi.nlm.nih.gov/geo/ (accessed on 2 May 2025)). The sequence of the m 6 A methylation site within the FOS 3′ untranslated region is highlighted. The asterisk symbol (*) indicates the methylated adenosine. ( b ) Renilla luciferase ( RLuc ) activity was measured in cell lysates from MCF7 cells transfected with the psiCHECK3-FOS construct and treated with ebselen. RLuc activity was normalized to Firefly luciferase activity ( FLuc ) ( upper panel ), and the corresponding mRNA expression in the lysates was analyzed using end-point PCR for the indicated target genes ( lower panel ). Data are presented as mean ± SD; n = 3. One-way ANOVA, ** , ## p < 0.01. ( c , d ) Ebselen reduces c-Fos translation efficiency. MCF7 cells were cultured in serum-free medium with indicated concentrations of ebselen for 24 h, followed by puromycin pretreatment for 1 h and EGF treatment for another 1 h. Whole cell lysates (WCLs) were analyzed via immunoprecipitation/immunoblotting (IP/IB) using the indicated antibodies ( c ). MCF7 cells transfected with sgControl or sgYTHDF1 were cultured in serum-free medium for 24 h, followed by pretreatment with puromycin for 1 h and EGF stimulation for another 1 h. WCLs were analyzed via IP/IB using the indicated antibodies ( d ).
Anthony Leung 32, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anthony leung 32/product/Addgene inc
Average 93 stars, based on 1 article reviews
anthony leung 32 - by Bioz Stars, 2026-06
93/100 stars
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eleutheroside e; acanthoside d  (TargetMol)
N/A
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salvileucantholide  (TargetMol)
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Regulation of FOS translation efficiency by ebselen. ( a ) Schematic representation of the psiCHECK3-FO translation reporter construct (upper) and Integrated Genomics Viewer plot showing m 6 A peaks around FOS mRNA in MCF7 cells (data from GSM5368774 , retrieved from https://www.ncbi.nlm.nih.gov/geo/ (accessed on 2 May 2025)). The sequence of the m 6 A methylation site within the FOS 3′ untranslated region is highlighted. The asterisk symbol (*) indicates the methylated adenosine. ( b ) Renilla luciferase ( RLuc ) activity was measured in cell lysates from MCF7 cells transfected with the psiCHECK3-FOS construct and treated with ebselen. RLuc activity was normalized to Firefly luciferase activity ( FLuc ) ( upper panel ), and the corresponding mRNA expression in the lysates was analyzed using end-point PCR for the indicated target genes ( lower panel ). Data are presented as mean ± SD; n = 3. One-way ANOVA, ** , ## p < 0.01. ( c , d ) Ebselen reduces c-Fos translation efficiency. MCF7 cells were cultured in serum-free medium with indicated concentrations of ebselen for 24 h, followed by puromycin pretreatment for 1 h and EGF treatment for another 1 h. Whole cell lysates (WCLs) were analyzed via immunoprecipitation/immunoblotting (IP/IB) using the indicated antibodies ( c ). MCF7 cells transfected with sgControl or sgYTHDF1 were cultured in serum-free medium for 24 h, followed by pretreatment with puromycin for 1 h and EGF stimulation for another 1 h. WCLs were analyzed via IP/IB using the indicated antibodies ( d ).

Journal: International Journal of Molecular Sciences

Article Title: Ebselen Suppresses Breast Cancer Tumorigenesis by Inhibiting YTHDF1-Mediated c-Fos Expression

doi: 10.3390/ijms26199416

Figure Lengend Snippet: Regulation of FOS translation efficiency by ebselen. ( a ) Schematic representation of the psiCHECK3-FO translation reporter construct (upper) and Integrated Genomics Viewer plot showing m 6 A peaks around FOS mRNA in MCF7 cells (data from GSM5368774 , retrieved from https://www.ncbi.nlm.nih.gov/geo/ (accessed on 2 May 2025)). The sequence of the m 6 A methylation site within the FOS 3′ untranslated region is highlighted. The asterisk symbol (*) indicates the methylated adenosine. ( b ) Renilla luciferase ( RLuc ) activity was measured in cell lysates from MCF7 cells transfected with the psiCHECK3-FOS construct and treated with ebselen. RLuc activity was normalized to Firefly luciferase activity ( FLuc ) ( upper panel ), and the corresponding mRNA expression in the lysates was analyzed using end-point PCR for the indicated target genes ( lower panel ). Data are presented as mean ± SD; n = 3. One-way ANOVA, ** , ## p < 0.01. ( c , d ) Ebselen reduces c-Fos translation efficiency. MCF7 cells were cultured in serum-free medium with indicated concentrations of ebselen for 24 h, followed by puromycin pretreatment for 1 h and EGF treatment for another 1 h. Whole cell lysates (WCLs) were analyzed via immunoprecipitation/immunoblotting (IP/IB) using the indicated antibodies ( c ). MCF7 cells transfected with sgControl or sgYTHDF1 were cultured in serum-free medium for 24 h, followed by pretreatment with puromycin for 1 h and EGF stimulation for another 1 h. WCLs were analyzed via IP/IB using the indicated antibodies ( d ).

Article Snippet: To design the translation reporter construct, the putative m 6 A modification site of FOS (chr4:5278828–75282230) was PCR-amplified from MCF7 cDNA and cloned into the 3′-UTR of RLuc at the XhoI site of the psiCHECK3 vector (a gift from Anthony Leung; Addgene plasmid #136010).

Techniques: Construct, Sequencing, Methylation, Luciferase, Activity Assay, Transfection, Expressing, Cell Culture, Immunoprecipitation, Western Blot